Wrongly submitted the GST

Bill cancelled is Purchase bill or its sale bill?
You cancelled the bill or opposite party cancelled the bill?

It's a transportation invoice

If it's transportation bill relating to expenses got cancelled, than you have to reverse the Input tax credit taken in month of feb 2020 in your current GSTR 3b return

It's service of truck provided on monthly basis and GSTR 3B return also files

Ok, if transportation service is provided by you ..and you already shown that invoice as output tax liability in GSTR 3B and In GSTR 1, and afterwards that invoice got cancelled, than you can do amendments in invoice in current GSTR 1 and also reduce the output tax liability in current GSTR 3B up to September 2020 return